connexin 62 CRISPR/Cas9 KO Plasmid (h): sc-405882
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 62 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the connexin 62 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 62 CRISPR/Cas9 KO Plasmid (h) | sc-405882 | 20 µg | $397.00 |
Overview
GJA10 encodes connexin 62, a gap junction channel subunit that assembles into connexons to enable direct intercellular exchange of ions and small metabolites. Connexin-mediated coupling supports electrical and metabolic synchronization, contributing to processes such as tissue homeostasis, cell-cycle coordination, and responses to cellular stress through regulated junctional permeability. Altered connexin expression or channel gating is widely linked to disrupted cell–cell communication, with downstream effects on differentiation programs and signaling networks including calcium dynamics and kinase-dependent pathways. As a member of the connexin family, connexin 62 provides a target for investigating how gap junction remodeling influences disease-relevant phenotypes in human cells.
connexin 62 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GJA10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GJA10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GJA10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish connexin 62 protein expression.
This CRISPR knockout system enables efficient generation of GJA10-deficient cell models for investigation of connexin 62 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GJA10 exon(s) critical for connexin 62 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GJA10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by connexin 62 CRISPR/Cas9 KO Plasmid (h) and connexin 62 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GJA10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by connexin 62 HDR Plasmid (h) and connexin 62 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GJA10 homology arms to support homology-directed repair at defined GJA10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.