COL18A1 CRISPR/Cas9 KO Plasmid (h): sc-402281
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL18A1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL18A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL18A1 Antibody (1837-46): sc-32720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL18A1 CRISPR/Cas9 KO Plasmid (h) | sc-402281 | 20 µg | $397.00 |
Overview
COL18A1 encodes collagen XVIII, a basement membrane–associated multiplexin collagen that contributes to extracellular matrix organization and tissue compartmentalization. Proteolytic processing of its C-terminal region generates endostatin, a bioactive fragment that modulates endothelial cell behavior and angiogenic signaling through interactions with heparan sulfate proteoglycans and integrins. Through its roles in basement membrane integrity and vascular homeostasis, COL18A1 influences cell adhesion, migration, and microenvironmental cues that shape development and wound remodeling. Genetic disruption of COL18A1 is linked to developmental and ocular phenotypes and is frequently studied in the context of angiogenesis, fibrosis, and tumor microenvironment biology.
COL18A1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COL18A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COL18A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COL18A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL18A1 protein expression.
This CRISPR knockout system enables efficient generation of COL18A1-deficient cell models for investigation of COL18A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COL18A1 exon(s) critical for COL18A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COL18A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL18A1 CRISPR/Cas9 KO Plasmid (h) and COL18A1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COL18A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL18A1 HDR Plasmid (h) and COL18A1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COL18A1 homology arms to support homology-directed repair at defined COL18A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.