COL17A1 CRISPR/Cas9 KO Plasmid (m): sc-419738
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL17A1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL17A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL17A1 CRISPR/Cas9 KO Plasmid (m) | sc-419738 | 20 µg | $397.00 |
Overview
Col17a1 encodes collagen type XVII alpha 1 (COL17A1), a transmembrane collagen and core component of hemidesmosomes that anchors basal keratinocytes to the underlying basement membrane. COL17A1 interacts with laminin-332 and integrin complexes to support epidermal adhesion, basement membrane organization, and skin appendage homeostasis, and its regulated ectodomain shedding influences keratinocyte migration and wound-associated remodeling. In mouse tissues, COL17A1 contributes to epithelial integrity and mechanotransduction at the dermal–epidermal junction, linking extracellular matrix cues to cytoskeletal stability. Disruption of COL17A1 function is broadly relevant to studies of skin fragility, blistering phenotypes, hair follicle cycling, and inflammation-associated epithelial barrier defects.
COL17A1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Col17a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Col17a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Col17a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL17A1 protein expression.
This CRISPR knockout system enables efficient generation of Col17a1-deficient cell models for investigation of COL17A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Col17a1 exon(s) critical for COL17A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Col17a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL17A1 CRISPR/Cas9 KO Plasmid (m) and COL17A1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Col17a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL17A1 HDR Plasmid (m) and COL17A1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Col17a1 homology arms to support homology-directed repair at defined Col17a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.