COL15A1 CRISPR/Cas9 KO Plasmid (h): sc-404027
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL15A1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL15A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL15A1 CRISPR/Cas9 KO Plasmid (h) | sc-404027 | 20 µg | $397.00 |
Overview
COL15A1 encodes collagen type XV alpha 1 chain, a non-fibrillar collagen localized to basement membrane and pericellular extracellular matrix where it supports tissue architecture and cell–matrix communication. By contributing to extracellular matrix assembly and remodeling, COL15A1 influences adhesion, migration, and mechanotransduction programs that intersect with integrin/FAK signaling and matrix-regulated growth factor availability. Altered COL15A1 expression is associated with changes in stromal organization, angiogenic niche behavior, and invasive phenotypes observed across multiple disease contexts, including cancer biology and vascular-associated pathologies. As an extracellular matrix component, COL15A1 is frequently studied for its role in microenvironmental regulation of epithelial and endothelial cell behavior.
COL15A1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COL15A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COL15A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COL15A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL15A1 protein expression.
This CRISPR knockout system enables efficient generation of COL15A1-deficient cell models for investigation of COL15A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COL15A1 exon(s) critical for COL15A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COL15A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL15A1 CRISPR/Cas9 KO Plasmid (h) and COL15A1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COL15A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL15A1 HDR Plasmid (h) and COL15A1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COL15A1 homology arms to support homology-directed repair at defined COL15A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.