CLK1 CRISPR/Cas9 KO Plasmid (m): sc-419688
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLK1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CLK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLK1 Antibody (F-12): sc-515897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLK1 CRISPR/Cas9 KO Plasmid (m) | sc-419688 | 20 µg | $397.00 |
Overview
Clk1 encodes CDC-like kinase 1 (CLK1), a dual-specificity protein kinase that phosphorylates serine/arginine-rich (SR) splicing factors and helps control spliceosome dynamics. Through modulation of alternative splicing and pre-mRNA processing, CLK1 contributes to transcriptional programs governing cell-cycle progression, differentiation, and stress-adaptive gene expression. CLK1 activity intersects with signaling networks that tune RNA processing, including pathways responsive to cellular stress and growth cues, thereby influencing proteome diversity. Dysregulated CLK family kinase activity and aberrant splicing patterns have been implicated in multiple disease-relevant phenotypes, making mouse Clk1 a useful node for mechanistic studies of RNA processing in development and pathology.
CLK1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Clk1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Clk1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Clk1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CLK1 protein expression.
This CRISPR knockout system enables efficient generation of Clk1-deficient cell models for investigation of CLK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Clk1 exon(s) critical for CLK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Clk1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CLK1 CRISPR/Cas9 KO Plasmid (m) and CLK1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Clk1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CLK1 HDR Plasmid (m) and CLK1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Clk1 homology arms to support homology-directed repair at defined Clk1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.