CLIP-170 Antibody (E-5): sc-48411

CLIP-170 Antibody (E-5) is a mouse monoclonal IgG2b antibody that detects CLIP-170 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLIP-170 is a crucial member of the plus-end-binding proteins (+TIPs) family, playing a significant role in microtubule dynamics by binding to growing plus ends of microtubules and linking them to organelle membranes. This interaction maintains cellular structure and function, as CLIP-170 acts as an anticatastrophic factor, promoting microtubule rescue, particularly near the cell periphery, which is essential for processes such as cell division and intracellular transport. CLIP-170′s unique ability to form comet-like structures at microtubule ends allows for real-time visualization of microtubule dynamics, providing insights into cellular behavior. Additionally, CLIP-170 co-localizes with key proteins such as Dynactin and Dynein at microtubule ends and the kinetochore, highlighting CLIP-170′s importance in the orchestration of cellular transport and mitotic processes. Notably, the splice variant of CLIP-170, known as Restin, is highly expressed in Hodgkin and Reed-Sternberg cells, monocyte-derived dendritic cells, IL-4 + CD40L activated B cells, and Ki-1 lymphoma, further underscoring CLIP-170′s relevance in both normal physiology and disease states.

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CLIP-170 Antibody (E-5) References:

1. CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family.  |  Perez, F., et al. 2002. J Cell Biol. 156: 631-42. PMID: 11854307
2. CLIP-170 interacts with dynactin complex and the APC-binding protein EB1 by different mechanisms.  |  Goodson, HV., et al. 2003. Cell Motil Cytoskeleton. 55: 156-73. PMID: 12789661
3. The microtubule-binding protein CLIP-170 coordinates mDia1 and actin reorganization during CR3-mediated phagocytosis.  |  Lewkowicz, E., et al. 2008. J Cell Biol. 183: 1287-98. PMID: 19114595
4. Pregnenolone activates CLIP-170 to promote microtubule growth and cell migration.  |  Weng, JH., et al. 2013. Nat Chem Biol. 9: 636-42. PMID: 23955365
5. Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment.  |  Kakeno, M., et al. 2014. Cell Struct Funct. 39: 45-59. PMID: 24451569
6. CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome alignment.  |  Amin, MA., et al. 2014. J Cell Sci. 127: 2818-24. PMID: 24777477
7. Overexpression of the microtubule-binding protein CLIP-170 induces a +TIP network superstructure consistent with a biomolecular condensate.  |  Wu, YO., et al. 2021. PLoS One. 16: e0260401. PMID: 34890409
8. The CLIP-170 N-terminal domain binds directly to both F-actin and microtubules in a mutually exclusive manner.  |  Wu, YO., et al. 2022. J Biol Chem. 298: 101820. PMID: 35283190
9. Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress.  |  Wang, X., et al. 2022. Nat Commun. 13: 5565. PMID: 36138017
10. CLIP170 enhancing FOSL1 expression via attenuating ubiquitin-mediated degradation of β-catenin drives renal cell carcinoma progression.  |  Huang, Y., et al. 2024. Cell Mol Life Sci. 81: 467. PMID: 39607512

The preferred CLIP-170 antibody is CLIP-170 Antibody (F-3): sc-28325.