Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Clathrin Light Chain B/CLTB Double Nickase Plasmid (h): sc-404228-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clathrin Light Chain B/CLTB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Clathrin Light Chain B/CLTB Double Nickase Plasmid (h) and Clathrin Light Chain B/CLTB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLTB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Clathrin Light Chain B/CLTB Antibody (G-7): sc-376414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clathrin Light Chain B/CLTB Double Nickase Plasmid (h) | sc-404228-NIC | 20 µg | $410.00 | |||
Clathrin Light Chain B/CLTB Double Nickase Plasmid (h2) | sc-404228-NIC-2 | 20 µg | $410.00 |
CLTB encodes clathrin light chain B, a core component of clathrin-coated vesicles that regulates assembly and curvature of clathrin lattices during endocytosis and post-Golgi trafficking. By modulating coated pit dynamics and adaptor interactions, CLTB contributes to receptor internalization, synaptic vesicle cycling, and sorting of membrane proteins across endosomal pathways. Clathrin-mediated transport influences signal transduction and nutrient uptake, linking CLTB-dependent vesicular traffic to cellular homeostasis and stress responses. Dysregulated endocytic trafficking and coated-vesicle function are implicated in cancer biology, neurobiology, and host–pathogen interactions, supporting CLTB as a useful node for mechanistic studies of membrane transport.
Clathrin Light Chain B/CLTB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLTB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLTB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLTB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLTB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.