CHCHD10 Double Nickase Plasmid (m): sc-430459-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHCHD10 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CHCHD10 Double Nickase Plasmid (m) and CHCHD10 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Chchd10. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHCHD10 Double Nickase Plasmid (m) | sc-430459-NIC | 20 µg | $410.00 |
Mouse Chchd10 encodes CHCHD10, a coiled-coil-helix-coiled-coil-helix domain protein that localizes to mitochondria and supports mitochondrial architecture and oxidative phosphorylation. It is implicated in maintaining cristae integrity, regulating respiratory chain performance, and coordinating mitochondrial stress responses that influence cellular energy homeostasis. CHCHD10 function intersects with mitochondrial quality control processes, including proteostasis and dynamics, which shape susceptibility to metabolic and neurodegenerative stress. Altered CHCHD10 biology has been associated with mitochondrial dysfunction phenotypes relevant to neuromuscular and neurodegeneration-focused research, making it useful for mechanistic studies of mitochondrial pathways.
CHCHD10 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Chchd10 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Chchd10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Chchd10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Chchd10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.