Cdc25B Antibody (DCS-162): sc-56266
- Cdc25B Antibody (DCS-162) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against full length Cdc25B of human origin
- Cdc25B Antibody (DCS-162) is recommended for detection of Cdc25B of human origin by IP, IF, IHC(P) and ELISA
- Anti-Cdc25B Antibody (DCS-162) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc25B Antibody (DCS-162) for IHC(P) applications. These reagents are now offered in bundles with Cdc25B Antibody (DCS-162) (see ordering information below).
Cdc25B Antibody (DCS-162) is a mouse monoclonal IgG1 kappa light chain antibody that detects human Cdc25B through immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Cdc25B antibody (DCS-162) is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes. Cdc25B functions as a phosphatase that dephosphorylates and activates cyclin-dependent kinase Cdc2, playing a crucial role in cell cycle regulation during G2 to mitosis transition. Proper activation of Cdc2 ensures timely and controlled cell division. Dysregulation of Cdc25B can promote uncontrolled cell proliferation and contribute to various cancers, making Cdc25B an important therapeutic target. Cdc25B contains a conserved catalytic domain essential for phosphatase activity and undergoes complex regulation through phosphorylation and protein-protein interactions, highlighting Cdc25B′s importance in cell cycle control.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc25B Antibody (DCS-162) References:
- Cdc25B as a steroid receptor coactivator. | Chua, SS., et al. 2004. Vitam Horm. 68: 231-56. PMID: 15193457
- The dual specificity phosphatase Cdc25B, but not the closely related Cdc25C, is capable of inhibiting cellular proliferation in a manner dependent upon its catalytic activity. | Varmeh-Ziaie, S. and Manfredi, JJ. 2007. J Biol Chem. 282: 24633-41. PMID: 17591782
- Differential mitotic degradation of the CDC25B phosphatase variants. | Kieffer, I., et al. 2007. Oncogene. 26: 7847-58. PMID: 17599046
- CDC25B involvement in the centrosome duplication cycle and in microtubule nucleation. | Boutros, R., et al. 2007. Cancer Res. 67: 11557-64. PMID: 18089784
- Asymmetric localization of the CDC25B phosphatase to the mother centrosome during interphase. | Boutros, R. and Ducommun, B. 2008. Cell Cycle. 7: 401-6. PMID: 18235220
- Moderate variations in CDC25B protein levels modulate the response to DNA damaging agents. | Aressy, B., et al. 2008. Cell Cycle. 7: 2234-40. PMID: 18635965
- Control of M-phase by maturation-promoting factor. | Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
- Direct activation of cdc2 with phosphatase: identification of p13suc1-sensitive and insensitive steps. | Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
- Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. | Gould, KL. and Nurse, P. 1989. Nature. 342: 39-45. PMID: 2682257
- Dominoes and clocks: the union of two views of the cell cycle. | Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
- CDC25B and CDC25C overexpression in nonmelanoma skin cancer suppresses cell death. | Al-Matouq, J., et al. 2019. Mol Carcinog. 58: 1691-1700. PMID: 31237025
- CDC25B Inhibition by Menadione: A Potential New Therapeutical Approach. | Ribeiro, HF., et al. 2022. Anticancer Agents Med Chem. 22: 2927-2932. PMID: 35440317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc25B Antibody (DCS-162) | sc-56266 | 200 µg/ml | $322.00 | |||
Cdc25B Antibody (DCS-162): m-IgG Fc BP-HRP Bundle | sc-526878 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cdc25B Antibody (DCS-162): m-IgG1 BP-HRP Bundle | sc-532251 | 200 µg Ab; 20 µg BP | $361.00 | |||
Cdc25B Antibody (DCS-162) AC | sc-56266 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc25B Antibody (DCS-162) HRP | sc-56266 HRP | 200 µg/ml | $322.00 | |||
Cdc25B Antibody (DCS-162) FITC | sc-56266 FITC | 200 µg/ml | $336.00 | |||
Cdc25B Antibody (DCS-162) PE | sc-56266 PE | 200 µg/ml | $349.00 | |||
Cdc25B Antibody (DCS-162) Alexa Fluor® 488 | sc-56266 AF488 | 200 µg/ml | $364.00 | |||
Cdc25B Antibody (DCS-162) Alexa Fluor® 546 | sc-56266 AF546 | 200 µg/ml | $364.00 | |||
Cdc25B Antibody (DCS-162) Alexa Fluor® 594 | sc-56266 AF594 | 200 µg/ml | $364.00 | |||
Cdc25B Antibody (DCS-162) Alexa Fluor® 647 | sc-56266 AF647 | 200 µg/ml | $364.00 | |||
Cdc25B Antibody (DCS-162) Alexa Fluor® 680 | sc-56266 AF680 | 200 µg/ml | $364.00 | |||
Cdc25B Antibody (DCS-162) Alexa Fluor® 790 | sc-56266 AF790 | 200 µg/ml | $364.00 |