CD98 Antibody (E-5): sc-376815

CD98 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD98 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CD98 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD98, also known as SLC3A2, is a disulfide-linked heterodimer consisting of a glycosylated heavy chain and a non-glycosylated light chain, which plays a crucial role as a scaffolding protein. CD98 is essential for interaction with basolaterally expressed amino acid transporters and β1 integrins, thereby influencing amino acid transport, cell adhesion, migration, and branching morphogenesis. CD98 heavy chain functions as a type II integral membrane protein and is predominantly expressed on T cells, where CD98 is upregulated upon T cell activation, as well as on monocytes, with lower expression levels found in granulocytes, platelets, and lymphocytes. CD98 is implicated in the regulation of T cell activation and proliferation, highlighting CD98′s significance in immune responses. Additionally, alternate transcriptional splice variants exist for the human CD98 gene, encoding different isoforms that may have distinct functional roles.

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CD98 Antibody (E-5) References:

1. Cross-linking CD98 promotes integrin-like signaling and anchorage-independent growth.  |  Rintoul, RC., et al. 2002. Mol Biol Cell. 13: 2841-52. PMID: 12181350
2. CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia.  |  Liu, X., et al. 2003. J Biol Chem. 278: 23672-7. PMID: 12716892
3. Activation of epithelial CD98 glycoprotein perpetuates colonic inflammation.  |  Kucharzik, T., et al. 2005. Lab Invest. 85: 932-41. PMID: 15880135
4. Ecto-phosphorylation of CD98 regulates cell-cell interactions.  |  Nguyen, HT., et al. 2008. PLoS One. 3: e3895. PMID: 19065266
5. MicroRNA-7 modulates CD98 expression during intestinal epithelial cell differentiation.  |  Nguyen, HT., et al. 2010. J Biol Chem. 285: 1479-89. PMID: 19892711
6. CD99 inhibits CD98-mediated β1 integrin signaling through SHP2-mediated FAK dephosphorylation.  |  Lee, KJ., et al. 2015. Exp Cell Res. 336: 211-22. PMID: 26172215
7. Integrative discovery of CD98 as a melanoma biomarker.  |  Theodosakis, N., et al. 2016. Pigment Cell Melanoma Res. 29: 385-7. PMID: 26850337
8. Prognostic impact of LAT1 and CD98 expression in cutaneous angiosarcoma.  |  Shimizu, A., et al. 2017. Neoplasma. 64: 283-288. PMID: 28052681
9. The CD98 Heavy Chain Is a Marker and Regulator of Head and Neck Squamous Cell Carcinoma Radiosensitivity.  |  Digomann, D., et al. 2019. Clin Cancer Res. 25: 3152-3163. PMID: 30670494
10. Early appearance of functional plasma cells (CD138+CD98+) in non-immunized neonate mice.  |  Orozco-Uribe, M., et al. 2023. Immunol Lett. 259: 9-20. PMID: 37225058

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD98 Antibody (E-5) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-CD98 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD98 Antibody (E-5) Specifications

Alternate Product Name: SLC3A2

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 230-529

Epitope Target: CD98

Epitope Species: human

Product Reactivity/Detection: CD98

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for CD98 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD98 antibody is CD98 Antibody (4F2): sc-59145.