Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD81 Double Nickase Plasmid (h): sc-400185-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD81 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD81 Double Nickase Plasmid (h) and CD81 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD81. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD81 Antibody (B-11): sc-166029
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD81 Double Nickase Plasmid (h) | sc-400185-NIC | 20 µg | $410.00 | |||
CD81 Double Nickase Plasmid (h2) | sc-400185-NIC-2 | 20 µg | $410.00 |
CD81 is a human tetraspanin (TAPA-1) that organizes tetraspanin-enriched microdomains on the plasma membrane and coordinates receptor clustering, adhesion, and signal transduction. Through interactions with partners such as CD19/CD21 in B cells and integrins including ITGA4/ITGB1, CD81 modulates immune cell activation, migration, and membrane trafficking processes. It also participates in vesicle biology and exosome composition, influencing intercellular communication and antigen presentation. Dysregulated CD81-associated networks have been linked to immune dysfunction and altered susceptibility to pathogen entry, making CD81 a useful target for studying membrane organization and host–pathogen interactions.
CD81 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD81 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD81. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD81 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD81-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.