Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
casein kinase IIα Double Nickase Plasmid (h): sc-418515-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- casein kinase IIα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- casein kinase IIα Double Nickase Plasmid (h) and casein kinase IIα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSNK2A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: casein kinase IIα Antibody (E-7): sc-373894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
casein kinase IIα Double Nickase Plasmid (h) | sc-418515-NIC | 20 µg | $410.00 | |||
casein kinase IIα Double Nickase Plasmid (h2) | sc-418515-NIC-2 | 20 µg | $410.00 |
CSNK2A1 encodes the catalytic alpha subunit of casein kinase II (CK2α), a constitutively active serine/threonine kinase that phosphorylates diverse substrates involved in signal transduction, transcriptional control, and protein turnover. CK2α functions in pathways governing cell-cycle progression, DNA damage responses, and stress signaling, and it modulates chromatin-associated processes through phosphorylation of nuclear factors. Dysregulated CSNK2A1/CK2 activity has been linked to altered growth signaling and aberrant phosphorylation networks observed across multiple disease contexts, including cancer and neurodevelopmental disorders. As a central node in phosphoregulation, CK2α is frequently studied to map kinase-dependent circuits and to define context-specific dependencies in human cells.
casein kinase IIα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSNK2A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSNK2A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSNK2A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSNK2A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.