Brn-3a/BRN3A/POU4F1 Antibody (14A6): sc-8429

Brn-3a Antibody (14A6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Brn-3a protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Brn-3a antibody (14A6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Brn-3a, a member of the Brn family of transcription factors, plays a crucial role in the development of the central nervous system by regulating the expression of genes necessary for neuronal differentiation and survival. Brn-3a (14A6) monoclonal antibody recognizes a highly restricted subset of neurons, where Brn-3a is essential for proper formation of sensory neurons and retinal development. Brn-3a′s unique structure includes two functional transactivating domains, which enhance transcription stimulation, making Brn-3a vital for target gene expression during early embryonic development. Understanding Brn-3a function and localization helps elucidate mechanisms underlying neurogenesis and potential implications in neurodegenerative diseases.

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Brn-3a/BRN3A/POU4F1 Antibody (14A6) References:

1. Brn-3a, a neuronal transcription factor of the POU gene family: indications for its involvement in cancer and angiogenesis.  |  Chiarugi, V., et al. 2002. Mol Biotechnol. 22: 123-7. PMID: 12405260
2. A novel POU homeodomain gene specifically expressed in cells of the developing mammalian nervous system.  |  Collum, RG., et al. 1992. Nucleic Acids Res. 20: 4919-25. PMID: 1357630
3. Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart.  |  Maskell, LJ., et al. 2017. Cell Death Dis. 8: e2861. PMID: 28594399
4. Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2.  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
5. Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2.  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
6. The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site.  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
7. POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells.  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
8. Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development.  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
9. Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors.  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
10. A single amino acid change converts an inhibitory transcription factor into an activator.  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
11. Mutation in transcription factor POU4F3 associated with inherited progressive hearing loss in humans.  |  Vahava, O., et al. 1998. Science. 279: 1950-4. PMID: 9506947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brn-3a/BRN3A/POU4F1 Antibody (14A6) has 157 citations in a variety of scientific publications.

Primary antibodies like Anti-Brn-3a/BRN3A/POU4F1 Antibody (14A6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brn-3a/BRN3A/POU4F1 Antibody (14A6) Specifications

Alternate Product Name: BRN3A

Product Clone Name: 14A6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-109

Epitope Target: Brn-3a

Epitope Species: mouse

Product Reactivity/Detection: Brn-3a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 157 citations in various scientific publications

Available Conjugates/Forms for Brn-3a/BRN3A/POU4F1 Antibody (14A6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent