Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Asparagine synthetase Double Nickase Plasmid (m): sc-424181-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Asparagine synthetase Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Asparagine synthetase Double Nickase Plasmid (m) and Asparagine synthetase Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Asns. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Asparagine synthetase Antibody (G-10): sc-365809
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Asparagine synthetase Double Nickase Plasmid (m) | sc-424181-NIC | 20 µg | $410.00 | |||
Asparagine synthetase Double Nickase Plasmid (m2) | sc-424181-NIC-2 | 20 µg | $410.00 |
Mouse Asns encodes asparagine synthetase, a cytosolic enzyme that catalyzes ATP-dependent conversion of aspartate and glutamine to asparagine and glutamate, sustaining intracellular asparagine pools and nitrogen homeostasis. ASNS activity supports amino acid biosynthesis and integrates with nutrient-sensing programs, including the integrated stress response and ATF4-regulated transcription during amino acid limitation. Perturbation of asparagine availability influences protein translation, redox balance, and metabolic rewiring, linking ASNS to cellular adaptation under nutrient stress. In biomedical research, Asns is frequently examined in contexts of metabolic vulnerability, stress signaling, and amino acid dependency phenotypes relevant to cancer cell metabolism and neurodevelopmental disorders.
Asparagine synthetase Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Asns locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Asns. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Asns function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Asns-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.