ASIC1 CRISPR/Cas9 KO Plasmid (h): sc-401452
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASIC1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ASIC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASIC1 CRISPR/Cas9 KO Plasmid (h) | sc-401452 | 20 µg | $397.00 |
Overview
ASIC1 (acid-sensing ion channel subunit 1) encodes a proton-gated, voltage-insensitive cation channel of the DEG/ENaC family that opens in response to extracellular acidification to permit Na⁺ influx and modulate membrane excitability. ASIC1 contributes to synaptic transmission, neuronal plasticity, and pH-dependent signaling, integrating with Ca²⁺-dependent pathways and activity-regulated gene programs in excitable cells. Altered ASIC1 activity has been linked to neuroinflammation and excitotoxic stress responses associated with ischemic and neurodegenerative contexts, and it is also studied in pain-related sensory processing. These features make human ASIC1 a useful target for dissecting acid-sensing mechanisms, ion homeostasis, and stimulus-coupled signaling networks.
ASIC1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ASIC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ASIC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ASIC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ASIC1 protein expression.
This CRISPR knockout system enables efficient generation of ASIC1-deficient cell models for investigation of ASIC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ASIC1 exon(s) critical for ASIC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ASIC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ASIC1 CRISPR/Cas9 KO Plasmid (h) and ASIC1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ASIC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ASIC1 HDR Plasmid (h) and ASIC1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ASIC1 homology arms to support homology-directed repair at defined ASIC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.