Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aquaporin 8/AQP8 CRISPR/Cas9 KO Plasmid (m): sc-419179
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 8/AQP8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aquaporin 8/AQP8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 8/AQP8 Antibody (14-Z): sc-81870
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 8/AQP8 CRISPR/Cas9 KO Plasmid (m) | sc-419179 | 20 µg | $397.00 |
Overview
Aqp8 encodes aquaporin 8 (AQP8), a membrane channel that facilitates rapid water movement and can also contribute to transmembrane transport of small neutral solutes such as hydrogen peroxide in selected cell types. In mouse tissues, AQP8 expression is associated with epithelial fluid handling and secretory physiology, and subcellular localization has been reported in both plasma membrane and intracellular membranes, supporting roles in osmotic balance and organelle homeostasis. By influencing water permeability and redox-related solute flux, AQP8 intersects with processes including epithelial barrier function, mitochondrial physiology, and oxidative stress signaling. Dysregulated AQP8 activity or expression has been investigated in the context of inflammatory and metabolic phenotypes, liver and gastrointestinal physiology, and broader perturbations of cellular redox homeostasis relevant to disease mechanisms.
Aquaporin 8/AQP8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aqp8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aqp8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aqp8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 8/AQP8 protein expression.
This CRISPR knockout system enables efficient generation of Aqp8-deficient cell models for investigation of Aquaporin 8/AQP8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aqp8 exon(s) critical for Aquaporin 8/AQP8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aqp8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aquaporin 8/AQP8 CRISPR/Cas9 KO Plasmid (m) and Aquaporin 8/AQP8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aqp8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aquaporin 8/AQP8 HDR Plasmid (m) and Aquaporin 8/AQP8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aqp8 homology arms to support homology-directed repair at defined Aqp8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.