Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Agrin CRISPR/Cas9 KO Plasmid (m): sc-419044
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Agrin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Agrin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Agrin Antibody (D-2): sc-374117
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Agrin CRISPR/Cas9 KO Plasmid (m) | sc-419044 | 20 µg | $397.00 |
Overview
Agrn encodes agrin, a large heparan sulfate proteoglycan of the extracellular matrix that organizes cell–matrix interactions and shapes specialized synaptic architecture. In mouse, agrin is best known for coordinating neuromuscular junction maturation through signaling networks that cluster acetylcholine receptors and stabilize pre- and postsynaptic differentiation, while also influencing basement membrane structure and neuronal connectivity. Beyond synapses, agrin contributes to mechanotransduction and adhesion-related pathways that affect cytoskeletal organization and tissue integrity. Dysregulated agrin biology has been linked to neuromuscular and neurodevelopmental phenotypes and to altered matrix remodeling contexts relevant to disease-associated tissue dysfunction.
Agrin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Agrn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Agrn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Agrn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Agrin protein expression.
This CRISPR knockout system enables efficient generation of Agrn-deficient cell models for investigation of Agrin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Agrn exon(s) critical for Agrin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Agrn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Agrin CRISPR/Cas9 KO Plasmid (m) and Agrin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Agrn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Agrin HDR Plasmid (m) and Agrin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Agrn homology arms to support homology-directed repair at defined Agrn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.