Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACSVL6 CRISPR/Cas9 KO Plasmid (h): sc-405327
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACSVL6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACSVL6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACSVL6 Antibody (C-8): sc-377374
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACSVL6 CRISPR/Cas9 KO Plasmid (h) | sc-405327 | 20 µg | $397.00 |
Overview
SLC27A5 encodes the human acyl-CoA synthetase very long-chain family member 6 (ACSVL6), an enzyme that activates long-chain fatty acids by converting them to acyl-CoA thioesters. This reaction supports lipid uptake and intracellular trafficking into mitochondrial and peroxisomal β-oxidation, while also supplying acyl-CoA substrates for triglyceride, phospholipid, and bile acid-related lipid remodeling. Through these roles, ACSVL6 contributes to metabolic homeostasis and intersects with pathways controlling energy balance, membrane composition, and lipid signaling. Altered fatty acid activation and downstream lipid flux are commonly implicated in metabolic dysfunction and liver-associated phenotypes, making SLC27A5 a useful target for mechanistic studies of lipid metabolism.
ACSVL6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC27A5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC27A5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC27A5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACSVL6 protein expression.
This CRISPR knockout system enables efficient generation of SLC27A5-deficient cell models for investigation of ACSVL6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC27A5 exon(s) critical for ACSVL6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC27A5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACSVL6 CRISPR/Cas9 KO Plasmid (h) and ACSVL6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC27A5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACSVL6 HDR Plasmid (h) and ACSVL6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC27A5 homology arms to support homology-directed repair at defined SLC27A5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.