Abi-2 Double Nickase Plasmid (m): sc-435933-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Abi-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Abi-2 Double Nickase Plasmid (m) and Abi-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Abi2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Abi-2 Antibody (B-3): sc-393982
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Abi-2 Double Nickase Plasmid (m) | sc-435933-NIC | 20 µg | $410.00 |
Mouse Abi2 encodes Abelson interactor 2 (Abi-2), an adaptor protein that regulates actin cytoskeleton remodeling and signal transduction downstream of receptor tyrosine kinases and small GTPases. Abi-2 functions within multiprotein complexes such as WAVE regulatory assemblies to coordinate Arp2/3-dependent actin polymerization, influencing lamellipodia formation, cell adhesion, and directed migration. Through these pathways, Abi-2 contributes to neuronal morphogenesis, immune cell trafficking, and epithelial dynamics, and altered Abi2-linked network activity has been associated with dysregulated proliferation and invasive phenotypes in disease-relevant models. Abi2 is therefore a useful node for studying cytoskeletal signaling, membrane protrusion dynamics, and pathway crosstalk that shapes cell behavior.
Abi-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Abi2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Abi2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Abi2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Abi2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.