ZNF598 Double Nickase Plasmid (h): sc-413866-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF598 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF598 Double Nickase Plasmid (h) and ZNF598 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZNF598. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF598 Double Nickase Plasmid (h) | sc-413866-NIC | 20 µg | $410.00 |
ZNF598 encodes an E3 ubiquitin ligase that functions as a key sensor and effector of ribosome-associated quality control, particularly during translation stalling and ribosome collisions. By ubiquitinating small ribosomal subunit proteins, ZNF598 promotes downstream events that resolve stalled translation complexes and coordinate mRNA and nascent polypeptide surveillance. This activity integrates with ubiquitin–proteasome signaling and translational stress responses that preserve proteostasis under conditions such as mRNA defects or oxidative stress. Dysregulation of ribosome quality control pathways is implicated in cellular vulnerability to proteotoxic and neurodegenerative stress, making ZNF598 a useful node for mechanistic studies of translational fidelity and stress adaptation.
ZNF598 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZNF598 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZNF598. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZNF598 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZNF598-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.