ZNF575 CRISPR/Cas9 KO Plasmid (m): sc-430383
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF575 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZNF575 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF575 CRISPR/Cas9 KO Plasmid (m) | sc-430383 | 20 µg | $397.00 |
Overview
Mouse Zfp575 encodes the zinc finger protein ZNF575, a putative DNA-binding transcriptional regulator within the C2H2-type zinc finger family that is often linked to sequence-specific control of gene expression. Although functional annotation remains limited, proteins of this class commonly modulate chromatin-associated transcriptional programs that influence cell state, differentiation, and responses to developmental or environmental cues. ZNF575 is therefore relevant for investigating gene regulatory networks and epigenetic mechanisms that shape tissue-specific transcription. Dysregulation of zinc finger–mediated transcriptional control is broadly associated with altered proliferation, genome stability, and disease-associated expression signatures, supporting exploratory studies of Zfp575 in these contexts.
ZNF575 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zfp575 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zfp575 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zfp575 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZNF575 protein expression.
This CRISPR knockout system enables efficient generation of Zfp575-deficient cell models for investigation of ZNF575 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zfp575 exon(s) critical for ZNF575 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zfp575 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZNF575 CRISPR/Cas9 KO Plasmid (m) and ZNF575 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zfp575 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZNF575 HDR Plasmid (m) and ZNF575 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zfp575 homology arms to support homology-directed repair at defined Zfp575 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.