ZNF526 Double Nickase Plasmid (m): sc-431684-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF526 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF526 Double Nickase Plasmid (m) and ZNF526 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zfp526. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF526 Double Nickase Plasmid (m) | sc-431684-NIC | 20 µg | $410.00 |
Zfp526 encodes the mouse zinc finger protein ZNF526, a putative nuclear DNA-binding factor implicated in transcriptional regulation through sequence-specific interactions with chromatin. As a multi–C2H2 zinc finger protein, ZNF526 is expected to participate in gene regulatory networks that influence cell state maintenance, lineage specification, and context-dependent transcriptional programs. Modulation of zinc finger–mediated transcriptional control intersects with pathways governing epigenetic regulation and developmental signaling, making Zfp526 a useful target for studying gene expression robustness and regulatory architecture. Dysregulation of zinc finger transcription factors is frequently associated with altered differentiation and proliferative phenotypes in disease-relevant models, supporting mechanistic investigation of Zfp526 in cellular homeostasis.
ZNF526 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zfp526 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zfp526. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zfp526 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zfp526-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.