ZMYM1 CRISPR/Cas9 KO Plasmid (h): sc-407092
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZMYM1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZMYM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZMYM1 CRISPR/Cas9 KO Plasmid (h) | sc-407092 | 20 µg | $397.00 |
Overview
ZMYM1 (zinc finger MYM-type protein 1) is a nuclear transcriptional regulator implicated in chromatin-dependent control of gene expression. Through its zinc-finger architecture and interactions with corepressor complexes, ZMYM1 contributes to transcriptional repression, maintenance of epigenetic states, and coordination of programs linked to cell-cycle progression and DNA damage responses. Dysregulated ZMYM1 activity has been associated with altered transcriptional networks observed in cancer and other disorders where chromatin regulation is perturbed, making it relevant for mechanistic studies of gene control in human cells.
ZMYM1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZMYM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZMYM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZMYM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZMYM1 protein expression.
This CRISPR knockout system enables efficient generation of ZMYM1-deficient cell models for investigation of ZMYM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZMYM1 exon(s) critical for ZMYM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZMYM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZMYM1 CRISPR/Cas9 KO Plasmid (h) and ZMYM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZMYM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZMYM1 HDR Plasmid (h) and ZMYM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZMYM1 homology arms to support homology-directed repair at defined ZMYM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.