ZFYVE28 CRISPR/Cas9 KO Plasmid (h): sc-412894
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZFYVE28 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZFYVE28 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZFYVE28 CRISPR/Cas9 KO Plasmid (h) | sc-412894 | 20 µg | $397.00 |
Overview
ZFYVE28 encodes a FYVE domain–containing protein that recognizes phosphatidylinositol 3-phosphate on endosomal membranes, linking phosphoinositide signaling to membrane trafficking and protein sorting. Through interactions with components of the endocytic machinery, ZFYVE28 is implicated in regulating receptor internalization, endosome maturation, and the spatial organization of signaling complexes. These processes intersect with pathways controlling growth factor receptor turnover and downstream signaling dynamics, making ZFYVE28 relevant to studies of signal attenuation and vesicular transport. Dysregulation of endosomal trafficking and phosphoinositide-dependent sorting is associated with altered cellular homeostasis and has been implicated across multiple disease-relevant contexts, including proliferative and neurobiological phenotypes.
ZFYVE28 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZFYVE28 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZFYVE28 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZFYVE28 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZFYVE28 protein expression.
This CRISPR knockout system enables efficient generation of ZFYVE28-deficient cell models for investigation of ZFYVE28 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZFYVE28 exon(s) critical for ZFYVE28 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZFYVE28 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZFYVE28 CRISPR/Cas9 KO Plasmid (h) and ZFYVE28 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZFYVE28 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZFYVE28 HDR Plasmid (h) and ZFYVE28 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZFYVE28 homology arms to support homology-directed repair at defined ZFYVE28 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.