Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZC3H7A CRISPR/Cas9 KO Plasmid (h): sc-412020
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZC3H7A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZC3H7A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZC3H7A Antibody (B-3): sc-398981
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZC3H7A CRISPR/Cas9 KO Plasmid (h) | sc-412020 | 20 µg | $397.00 |
Overview
ZC3H7A (zinc finger CCCH-type containing 7A) encodes an RNA-binding protein characterized by CCCH-type zinc finger motifs and functions in post-transcriptional gene regulation. It has been implicated in coordinating mRNA stability and translation through interactions with ribonucleoprotein complexes, supporting proper control of gene expression programs during cellular stress responses and proliferation. ZC3H7A has also been linked to viral–host interaction networks, where modulation of RNA metabolism can influence innate immune signaling and pathogen replication. Dysregulated RNA processing and stress-adaptive translation are recurring features of cancer and inflammatory disorders, making ZC3H7A a useful node for mechanistic studies of RNA-centric regulatory pathways.
ZC3H7A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZC3H7A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZC3H7A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZC3H7A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZC3H7A protein expression.
This CRISPR knockout system enables efficient generation of ZC3H7A-deficient cell models for investigation of ZC3H7A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZC3H7A exon(s) critical for ZC3H7A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZC3H7A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZC3H7A CRISPR/Cas9 KO Plasmid (h) and ZC3H7A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZC3H7A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZC3H7A HDR Plasmid (h) and ZC3H7A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZC3H7A homology arms to support homology-directed repair at defined ZC3H7A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.