Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
YY1 Double Nickase Plasmid (h): sc-400395-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- YY1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- YY1 Double Nickase Plasmid (h) and YY1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting YY1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: YY1 Antibody (H-10): sc-7341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
YY1 Double Nickase Plasmid (h) | sc-400395-NIC | 20 µg | $410.00 | |||
YY1 Double Nickase Plasmid (h2) | sc-400395-NIC-2 | 20 µg | $410.00 |
YY1 (Yin Yang 1) encodes a multifunctional zinc finger transcription factor that can act as both a transcriptional activator and repressor, coordinating context-dependent gene expression programs. YY1 participates in chromatin remodeling and epigenetic regulation through interactions with Polycomb-associated complexes and histone-modifying enzymes, influencing promoter/enhancer activity and 3D genome organization. It contributes to core cellular processes including cell-cycle progression, DNA damage responses, differentiation, and metabolic control. Dysregulated YY1 activity has been linked to altered transcriptional networks observed across diverse cancers and developmental disorders, making it a widely used node for investigating transcriptional control mechanisms.
YY1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the YY1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within YY1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt YY1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of YY1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.