YSK1 Antibody (E-5): sc-271196
- YSK1 Antibody (E-5) is a mouse monoclonal IgG1 κ YSK1 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against a peptide mapping at the N-terminus of YSK1 of human origin
- YSK1 Antibody (E-5) is recommended for detection of YSK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and bovine
- Anti-YSK1 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for YSK1 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with YSK1 Antibody (E-5) (see ordering information below).
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SEE ALSO...
YSK1 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects YSK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-YSK1 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. YSK1 protein, also known as yeast SPS/STE20-related kinase 1, plays a crucial role in cellular signaling pathways, particularly in the regulation of cell growth and differentiation. YSK1 is primarily located in the cytoplasm and has been shown to interact with several other proteins, including mammalian STE20-like kinases such as KHS, GLK, NIK, HPK1, Krs-1, Krs-2, and GC kinase. These interactions are significant as they highlight YSK1′s involvement in complex signaling networks that relay information from G-protein coupled receptors, ultimately influencing various cellular responses. YSK1′s unique property is that overexpression does not activate the SAPK/JNK pathway, distinguishing YSK1 from other STE20-like kinases and underscoring a specialized function in cellular signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
YSK1 Antibody (E-5) References:
- The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. | Leberer, E., et al. 1992. EMBO J. 11: 4815-24. PMID: 1464311
- Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. | Wu, C., et al. 1995. J Biol Chem. 270: 15984-92. PMID: 7608157
- NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain. | Su, YC., et al. 1997. EMBO J. 16: 1279-90. PMID: 9135144
- YSK1, a novel mammalian protein kinase structurally related to Ste20 and SPS1, but is not involved in the known MAPK pathways. | Osada, S., et al. 1997. Oncogene. 14: 2047-57. PMID: 9160885
- Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase. | Diener, K., et al. 1997. Proc Natl Acad Sci U S A. 94: 9687-92. PMID: 9275185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
YSK1 Antibody (E-5) | sc-271196 | 200 µg/ml | $322.00 | |||
YSK1 Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-529136 | 200 µg Ab; 10 µg BP | $361.00 | |||
YSK1 Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-521872 | 200 µg Ab, 40 µg BP | $361.00 | |||
YSK1 Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-543342 | 200 µg Ab; 20 µg BP | $361.00 | |||
YSK1 Antibody (E-5) AC | sc-271196 AC | 500 µg/ml, 25% agarose | $424.00 | |||
YSK1 Antibody (E-5) HRP | sc-271196 HRP | 200 µg/ml | $322.00 | |||
YSK1 Antibody (E-5) FITC | sc-271196 FITC | 200 µg/ml | $336.00 | |||
YSK1 Antibody (E-5) PE | sc-271196 PE | 200 µg/ml | $349.00 | |||
YSK1 Antibody (E-5) Alexa Fluor® 488 | sc-271196 AF488 | 200 µg/ml | $364.00 | |||
YSK1 Antibody (E-5) Alexa Fluor® 546 | sc-271196 AF546 | 200 µg/ml | $364.00 | |||
YSK1 Antibody (E-5) Alexa Fluor® 594 | sc-271196 AF594 | 200 µg/ml | $364.00 | |||
YSK1 Antibody (E-5) Alexa Fluor® 647 | sc-271196 AF647 | 200 µg/ml | $364.00 | |||
YSK1 Antibody (E-5) Alexa Fluor® 680 | sc-271196 AF680 | 200 µg/ml | $364.00 | |||
YSK1 Antibody (E-5) Alexa Fluor® 790 | sc-271196 AF790 | 200 µg/ml | $364.00 | |||
YSK1 (E-5) Neutralizing Peptide | sc-271196 P | 100 µg/0.5 ml | $69.00 |