VpreB Antibody (H-11): sc-514957
- VpreB Antibody (H-11) is a mouse monoclonal IgG1 κ VpreB antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 91-145 mapping at the C-terminus of VpreB of human origin
- VpreB Antibody (H-11) is recommended for detection of VpreB of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-VpreB Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VpreB Antibody (H-11) for WB applications. These reagents are now offered in bundles with VpreB Antibody (H-11) (see ordering information below).
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VpreB Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects VpreB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VpreB (H-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VpreB protein, also known as CD179a or pre-B lymphocyte 1, plays a crucial role in B cell development, specifically in pre-B lymphocytes, where VpreB is expressed but absent in mature B cells and other blood cell lineages. VpreB protein is essential for forming a molecular complex with the Ig-mu chain, which regulates immunoglobulin gene rearrangements during early B cell differentiation. The gene encoding VpreB is located on human chromosome 22q11.2, and in mice, VpreB and lambda 5 genes are co-expressed in pre-B cells as part of the same transcription unit, allowing for production of a primary transcript that undergoes alternative splicing to yield pre-B and lambda 5 mRNAs. In humans, these genes are separate entities, highlighting evolutionary differences in B cell development between species. The unique expression pattern and functional significance of VpreB make anti-VpreB antibody (H-11) an invaluable tool for studying B cell biology and related immunological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VpreB Antibody (H-11) References:
- The VpreB protein of the surrogate light-chain can pair with some mu heavy-chains in the absence of the lambda 5 protein. | Seidl, T., et al. 2001. Eur J Immunol. 31: 1999-2006. PMID: 11449352
- Analysis of VpreB expression during B lineage differentiation in lambda5-deficient mice. | Stephan, RP., et al. 2001. J Immunol. 167: 3734-9. PMID: 11564789
- The VpreB1 enhancer drives developmental stage-specific gene expression in vivo. | Licence, S., et al. 2003. Eur J Immunol. 33: 1117-26. PMID: 12672078
- Variable domains and a VpreB-like molecule are present in a jawless vertebrate. | Cannon, JP., et al. 2005. Immunogenetics. 56: 924-9. PMID: 15650874
- The pre-B-cell receptor induces silencing of VpreB and lambda5 transcription. | Parker, MJ., et al. 2005. EMBO J. 24: 3895-905. PMID: 16281060
- The human pre-B-specific lambda-like cluster is located in the 22q11.2-22q12.3 region, distal to the IgC lambda locus. | Mattei, MG., et al. 1991. Genomics. 9: 544-6. PMID: 1903358
- A second gene, VpreB in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes. | Kudo, A. and Melchers, F. 1987. EMBO J. 6: 2267-72. PMID: 3117530
- The omega and iota surrogate immunoglobulin light chains. | Pillai, S. and Baltimore, D. 1988. Curr Top Microbiol Immunol. 137: 136-9. PMID: 3138073
- The human Vpre B gene is located on chromosome 22 near a cluster of V lambda gene segments. | Bauer, SR., et al. 1988. Immunogenetics. 28: 328-33. PMID: 3139558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VpreB Antibody (H-11) | sc-514957 | 200 µg/ml | $322.00 | |||
VpreB Antibody (H-11): m-IgG Fc BP-HRP Bundle | sc-531252 | 200 µg Ab; 10 µg BP | $361.00 | |||
VpreB Antibody (H-11): m-IgGκ BP-HRP Bundle | sc-524885 | 200 µg Ab, 40 µg BP | $361.00 | |||
VpreB Antibody (H-11): m-IgG1 BP-HRP Bundle | sc-544454 | 200 µg Ab; 20 µg BP | $361.00 | |||
VpreB Antibody (H-11) AC | sc-514957 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VpreB Antibody (H-11) HRP | sc-514957 HRP | 200 µg/ml | $322.00 | |||
VpreB Antibody (H-11) FITC | sc-514957 FITC | 200 µg/ml | $336.00 | |||
VpreB Antibody (H-11) PE | sc-514957 PE | 200 µg/ml | $349.00 | |||
VpreB Antibody (H-11) Alexa Fluor® 488 | sc-514957 AF488 | 200 µg/ml | $364.00 | |||
VpreB Antibody (H-11) Alexa Fluor® 546 | sc-514957 AF546 | 200 µg/ml | $364.00 | |||
VpreB Antibody (H-11) Alexa Fluor® 594 | sc-514957 AF594 | 200 µg/ml | $364.00 | |||
VpreB Antibody (H-11) Alexa Fluor® 647 | sc-514957 AF647 | 200 µg/ml | $364.00 | |||
VpreB Antibody (H-11) Alexa Fluor® 680 | sc-514957 AF680 | 200 µg/ml | $364.00 | |||
VpreB Antibody (H-11) Alexa Fluor® 790 | sc-514957 AF790 | 200 µg/ml | $364.00 |