VMAT 2 Lentiviral Activation Particles (h): sc-401308-LAC
- Target species: human
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- VMAT 2 Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- VMAT 2 Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by VMAT 2 Lentiviral Activation Plasmid (h) and VMAT 2 Lentiviral Activation Plasmid (h2) target distinct regulatory regions of the SLC18A2 promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: VMAT 2 Antibody (H-12): sc-374079
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VMAT 2 Lentiviral Activation Particles (h) | sc-401308-LAC | 200 µl | $455.00 |
SLC18A2 encodes vesicular monoamine transporter 2 (VMAT2), an integral synaptic vesicle membrane protein that uses the proton electrochemical gradient to load dopamine, serotonin, norepinephrine, epinephrine, and histamine into secretory vesicles. By regulating vesicular sequestration and quantal release, VMAT2 controls monoaminergic neurotransmission and protects cells from cytosolic monoamine oxidation, linking it to vesicle trafficking, neurotransmitter metabolism, and oxidative stress pathways. Altered SLC18A2 expression or function has been associated with dopaminergic neuron vulnerability and neuropsychiatric phenotypes, making it relevant to studies of Parkinson’s disease mechanisms, mood regulation, and substance use biology. Human VMAT2 is also used as a marker and functional node in models of catecholaminergic and serotonergic differentiation and synaptic vesicle dynamics.
VMAT 2 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient SLC18A2 upregulation across a broader range of human cell types.
VMAT 2 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the SLC18A2 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous VMAT 2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native SLC18A2 genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.