Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USP22 CRISPR/Cas9 KO Plasmid (m): sc-432127
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP22 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP22 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP22 Antibody (C-3): sc-390585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP22 CRISPR/Cas9 KO Plasmid (m) | sc-432127 | 20 µg | $397.00 |
Overview
Usp22 encodes the deubiquitinating enzyme USP22, a catalytic component of the SAGA transcriptional coactivator complex that removes ubiquitin from histone H2B and other substrates to influence chromatin accessibility and gene expression programs. Through regulation of transcriptional elongation, cell-cycle progression, and DNA damage responses, USP22 helps coordinate proliferation, differentiation, and maintenance of genome integrity. USP22 activity intersects with epigenetic control pathways and ubiquitin-dependent proteostasis, shaping context-specific signaling outputs that are frequently altered in cancer-relevant transcriptional states. Dysregulated USP22-associated gene expression signatures have been linked to tumor aggressiveness and stem-like phenotypes, supporting its utility in mechanistic studies of oncogenic transcription and epigenetic plasticity.
USP22 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usp22 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usp22 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usp22 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP22 protein expression.
This CRISPR knockout system enables efficient generation of Usp22-deficient cell models for investigation of USP22 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usp22 exon(s) critical for USP22 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usp22 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP22 CRISPR/Cas9 KO Plasmid (m) and USP22 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usp22 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP22 HDR Plasmid (m) and USP22 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usp22 homology arms to support homology-directed repair at defined Usp22 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.