USP12 CRISPR/Cas9 KO Plasmid (h): sc-406586
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP12 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP12 CRISPR/Cas9 KO Plasmid (h) | sc-406586 | 20 µg | $397.00 |
Overview
USP12 (ubiquitin specific peptidase 12) is a deubiquitinating enzyme that regulates protein stability and signaling by removing ubiquitin chains from selected substrates. It functions in ubiquitin-dependent control of cellular homeostasis, influencing processes such as receptor trafficking, stress responses, and proteostasis through modulation of ubiquitin–proteasome system dynamics. USP12 activity has been linked to regulation of key signaling nodes including pathways associated with AKT and androgen receptor signaling, connecting it to context-dependent control of proliferation and transcriptional programs. Dysregulation of USP12 expression or function has been associated with tumor biology and other disorders where altered ubiquitin editing perturbs signaling amplitude and protein turnover.
USP12 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the USP12 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the USP12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the USP12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP12 protein expression.
This CRISPR knockout system enables efficient generation of USP12-deficient cell models for investigation of USP12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting USP12 exon(s) critical for USP12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple USP12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP12 CRISPR/Cas9 KO Plasmid (h) and USP12 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the USP12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP12 HDR Plasmid (h) and USP12 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by USP12 homology arms to support homology-directed repair at defined USP12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.