UDP-GlcDH Double Nickase Plasmid (h): sc-405002-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UDP-GlcDH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UDP-GlcDH Double Nickase Plasmid (h) and UDP-GlcDH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UGDH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UDP-GlcDH Antibody (B-4): sc-137058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UDP-GlcDH Double Nickase Plasmid (h) | sc-405002-NIC | 20 µg | $410.00 | |||
UDP-GlcDH Double Nickase Plasmid (h2) | sc-405002-NIC-2 | 20 µg | $410.00 |
Human UGDH encodes UDP-glucose 6-dehydrogenase (UDP-GlcDH), a cytosolic enzyme that oxidizes UDP-glucose to UDP-glucuronic acid, a central precursor for glycosaminoglycan and proteoglycan biosynthesis. By controlling UDP-glucuronic acid availability, UDP-GlcDH helps regulate extracellular matrix composition, glycocalyx architecture, and cell–cell/ cell–matrix interactions that influence adhesion, migration, and mechanotransduction. UGDH activity interfaces with carbohydrate metabolism and nucleotide-sugar interconversion pathways that support glycosylation programs during development and tissue remodeling. Dysregulated UGDH expression or flux through UDP-glucuronic acid can alter hyaluronan and sulfated GAG production, linking this axis to fibrosis, inflammatory signaling, and cancer-associated matrix reprogramming in experimental systems.
UDP-GlcDH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UGDH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UGDH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UGDH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UGDH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.