UCH-L5 Antibody (C-4): sc-271002
- UCH-L5 Antibody (C-4) is a mouse monoclonal IgG1 κ UCH-L5 antibody, cited in 12 publications, provided at 200 µg/ml
- raised against amino acids 220-329 mapping at the C-terminus of UCH-L5 of human origin
- UCH-L5 Antibody (C-4) is recommended for detection of UCH-L5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-UCH-L5 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for UCH-L5 Antibody (C-4) for WB applications. This reagent is now offered in a bundle with UCH-L5 Antibody (C-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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UCH-L5 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects UCH-L5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-UCH-L5 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UCH-L5, also known as ubiquitin carboxyl-terminal hydrolase isozyme L5 or UCH37, is a 329 amino acid protein that plays a crucial role in the 19S regulatory complex of the 26S proteasome, where UCH-L5 functions to edit polyubiquitinated protein substrates. UCH-L5′s ability to rescue ubiquitinated proteins, including those with oncogenic potential, from proteasomal degradation highlights UCH-L5′s importance in cellular regulation and tumor biology. Notably, UCH-L5 interacts with Smad7, leading to a significant upregulation of TGFβ-dependent gene expression through the de-ubiquitination and stabilization of TGFβ receptor I. Given that overexpression of UCH-L5 and other deubiquitinating enzymes has been observed in various cancer cell lines, targeting these proteins may offer promising avenues for cancer therapy. Additionally, UCH-L5 exists in four isoforms due to alternative splicing, which may contribute to UCH-L5′s functional diversity and regulatory mechanisms within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UCH-L5 Antibody (C-4) References:
- Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. | Lai, CH., et al. 2000. Genome Res. 10: 703-13. PMID: 10810093
- Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. | Yao, T., et al. 2006. Nat Cell Biol. 8: 994-1002. PMID: 16906146
- A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes. | Hamazaki, J., et al. 2006. EMBO J. 25: 4524-36. PMID: 16990800
- Reversible ubiquitination regulates the Smad/TGF-beta signalling pathway. | Wicks, SJ., et al. 2006. Biochem Soc Trans. 34: 761-3. PMID: 17052192
- A substrate for deubiquitinating enzymes based on time-resolved fluorescence resonance energy transfer between terbium and yellow fluorescent protein. | Horton, RA., et al. 2007. Anal Biochem. 360: 138-43. PMID: 17118327
- hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37. | Qiu, XB., et al. 2006. EMBO J. 25: 5742-53. PMID: 17139257
- New insights into the role of the ubiquitin-proteasome pathway in the regulation of apoptosis. | Liu, CH., et al. 2007. Chang Gung Med J. 30: 469-79. PMID: 18350730
- Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex. | Yao, T., et al. 2008. Mol Cell. 31: 909-17. PMID: 18922472
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UCH-L5 Antibody (C-4) | sc-271002 | 200 µg/ml | $322.00 | |||
UCH-L5 Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-521814 | 200 µg Ab, 40 µg BP | $361.00 | |||
UCH-L5 Antibody (C-4) AC | sc-271002 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UCH-L5 Antibody (C-4) HRP | sc-271002 HRP | 200 µg/ml | $322.00 | |||
UCH-L5 Antibody (C-4) FITC | sc-271002 FITC | 200 µg/ml | $336.00 | |||
UCH-L5 Antibody (C-4) PE | sc-271002 PE | 200 µg/ml | $349.00 | |||
UCH-L5 Antibody (C-4) Alexa Fluor® 488 | sc-271002 AF488 | 200 µg/ml | $364.00 | |||
UCH-L5 Antibody (C-4) Alexa Fluor® 546 | sc-271002 AF546 | 200 µg/ml | $364.00 | |||
UCH-L5 Antibody (C-4) Alexa Fluor® 594 | sc-271002 AF594 | 200 µg/ml | $364.00 | |||
UCH-L5 Antibody (C-4) Alexa Fluor® 647 | sc-271002 AF647 | 200 µg/ml | $364.00 | |||
UCH-L5 Antibody (C-4) Alexa Fluor® 680 | sc-271002 AF680 | 200 µg/ml | $364.00 | |||
UCH-L5 Antibody (C-4) Alexa Fluor® 790 | sc-271002 AF790 | 200 µg/ml | $364.00 |