Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRPC3/6/7 CRISPR/Cas9 KO Plasmid (h): sc-403740
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRPC3/6/7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRPC3/6/7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRPC6 Antibody (B-10): sc-515837
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRPC3/6/7 CRISPR/Cas9 KO Plasmid (h) | sc-403740 | 20 µg | $397.00 |
Overview
TRPC3 is a Ca2+-permeable, nonselective cation channel of the TRPC3/6/7 subfamily that couples receptor-activated phospholipase C signaling to membrane depolarization and intracellular calcium entry. Channel activity is regulated by diacylglycerol and integrates signals downstream of GPCR and receptor tyrosine kinase pathways, shaping Ca2+-dependent transcription, cytoskeletal remodeling, secretion, and contractility. TRPC3-containing channel complexes contribute to neuronal excitability and synaptic signaling, as well as smooth muscle and cardiac calcium handling through cross-talk with calcineurin/NFAT and MAPK pathways. Dysregulated TRPC3/6/7 axis has been linked to aberrant Ca2+ signaling in cardiovascular and neurodegenerative disease contexts and is frequently interrogated in models of hypertrophy, fibrosis, and excitotoxic stress.
TRPC3/6/7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRPC3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRPC3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRPC3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRPC3/6/7 protein expression.
This CRISPR knockout system enables efficient generation of TRPC3-deficient cell models for investigation of TRPC3/6/7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRPC3 exon(s) critical for TRPC3/6/7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRPC3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRPC3/6/7 CRISPR/Cas9 KO Plasmid (h) and TRPC3/6/7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRPC3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRPC3/6/7 HDR Plasmid (h) and TRPC3/6/7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRPC3 homology arms to support homology-directed repair at defined TRPC3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.