TFIIIC110 Double Nickase Plasmid (h): sc-404261-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFIIIC110 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TFIIIC110 Double Nickase Plasmid (h) and TFIIIC110 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GTF3C2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TFIIIC110 Antibody (2395C2a): sc-81406
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIIC110 Double Nickase Plasmid (h) | sc-404261-NIC | 20 µg | $410.00 | |||
TFIIIC110 Double Nickase Plasmid (h2) | sc-404261-NIC-2 | 20 µg | $410.00 |
GTF3C2 encodes TFIIIC110, a core subunit of the TFIIIC complex that recognizes internal promoter elements in RNA polymerase III–transcribed genes, including tRNAs and other small noncoding RNAs. By supporting TFIIIB recruitment and assembly of the Pol III preinitiation complex, TFIIIC110 contributes to basal transcription programs that couple RNA metabolism with cell growth and stress responses. TFIIIC also interfaces with chromatin organization and nuclear architecture, linking Pol III loci to broader genome regulatory features. Dysregulation of Pol III transcriptional control and TFIIIC subunits has been associated with proliferative states and altered small-RNA homeostasis, making GTF3C2 a useful target for mechanistic studies of transcription and genome regulation.
TFIIIC110 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GTF3C2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GTF3C2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GTF3C2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GTF3C2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.