Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Synapsin Ia/b CRISPR/Cas9 KO Plasmid (m): sc-423233
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synapsin Ia/b CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synapsin Ia/b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synapsin Ia/b Antibody (A-8): sc-376623
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synapsin Ia/b CRISPR/Cas9 KO Plasmid (m) | sc-423233 | 20 µg | $397.00 |
Overview
Syn1 encodes synapsin Ia/b, a neuron-specific phosphoprotein that associates with synaptic vesicles and the actin cytoskeleton to regulate vesicle clustering, reserve pool maintenance, and activity-dependent neurotransmitter release. Synapsin Ia/b is modulated by calcium- and cAMP-dependent phosphorylation cascades, linking neuronal firing to synaptic vesicle mobilization and presynaptic plasticity. Through these processes, Syn1 contributes to circuit maturation, synapse stability, and short-term synaptic plasticity that shape information processing in the central nervous system. Altered synapsin signaling has been implicated in neurodevelopmental and neuropsychiatric phenotypes and is widely studied in relation to seizure susceptibility and synaptic dysfunction.
Synapsin Ia/b CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syn1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syn1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syn1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synapsin Ia/b protein expression.
This CRISPR knockout system enables efficient generation of Syn1-deficient cell models for investigation of Synapsin Ia/b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syn1 exon(s) critical for Synapsin Ia/b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syn1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synapsin Ia/b CRISPR/Cas9 KO Plasmid (m) and Synapsin Ia/b CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syn1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synapsin Ia/b HDR Plasmid (m) and Synapsin Ia/b HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syn1 homology arms to support homology-directed repair at defined Syn1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.