Sox2 Double Nickase Plasmid (h): sc-400050-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sox2 Double Nickase Plasmid (h) and Sox2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SOX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox2 Antibody (E-4): sc-365823
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox2 Double Nickase Plasmid (h) | sc-400050-NIC | 20 µg | $410.00 | |||
Sox2 Double Nickase Plasmid (h2) | sc-400050-NIC-2 | 20 µg | $410.00 |
SOX2 encodes the transcription factor Sox2, a core regulator of pluripotency and lineage specification that maintains stem cell self-renewal while constraining differentiation programs. Sox2 cooperates with OCT4 and NANOG to control enhancer and promoter networks governing chromatin accessibility, transcriptional initiation, and cell fate decisions during early development. In neural and epithelial contexts, SOX2 influences progenitor maintenance and interacts with signaling pathways such as WNT, SHH, and Notch to modulate differentiation trajectories. Dysregulated SOX2 expression or genomic alteration is associated with developmental disorders and is frequently implicated in oncogenic transcriptional circuits, supporting its broad relevance in disease biology.
Sox2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SOX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SOX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SOX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SOX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.