Sox-5 Lentiviral Activation Particles (h): sc-401854-LAC

SOX5 encodes the transcription factor Sox-5, a member of the SRY-related HMG-box family that binds DNA and shapes lineage-specific gene expression programs. In human cells, Sox-5 contributes to developmental patterning and differentiation processes, including chondrogenic and neural transcriptional networks, by coordinating enhancer activity and chromatin-dependent regulation. Its activity intersects with pathways governing cell fate commitment and maturation, making SOX5 a useful node for studying regulatory circuitry during development and tissue homeostasis. Dysregulated SOX5 expression has been associated with neurodevelopmental phenotypes and altered transcriptional states reported across several disease contexts, supporting its relevance for mechanistic studies of gene-regulatory imbalance.

Sox-5 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient SOX5 upregulation across a broader range of human cell types.

Sox-5 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the SOX5 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Sox-5 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native SOX5 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.