SLC25A18 CRISPR/Cas9 KO Plasmid (h): sc-413500
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC25A18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLC25A18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC25A18 CRISPR/Cas9 KO Plasmid (h) | sc-413500 | 20 µg | $397.00 |
Overview
SLC25A18 encodes a mitochondrial inner membrane carrier of the SLC25 family implicated in transporting glutamate across the mitochondrial membrane, supporting nitrogen flux between cytosol and mitochondria. By modulating mitochondrial amino acid availability, SLC25A18 contributes to bioenergetic homeostasis, redox balance, and anaplerotic feeding of the TCA cycle, thereby influencing cellular responses to nutrient stress. Altered glutamate handling at the mitochondria is relevant to metabolic remodeling and oxidative stress pathways frequently studied in cancer biology and neurometabolic dysfunction. SLC25A18 is therefore used as a probe for mitochondrial substrate transport, amino acid metabolism, and mitochondria-centered signaling networks.
SLC25A18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC25A18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC25A18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC25A18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLC25A18 protein expression.
This CRISPR knockout system enables efficient generation of SLC25A18-deficient cell models for investigation of SLC25A18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC25A18 exon(s) critical for SLC25A18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC25A18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLC25A18 CRISPR/Cas9 KO Plasmid (h) and SLC25A18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC25A18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLC25A18 HDR Plasmid (h) and SLC25A18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC25A18 homology arms to support homology-directed repair at defined SLC25A18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.