Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SERCA1 Double Nickase Plasmid (h): sc-401416-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SERCA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SERCA1 Double Nickase Plasmid (h) and SERCA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP2A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SERCA1 Antibody (A-6): sc-515162
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SERCA1 Double Nickase Plasmid (h) | sc-401416-NIC | 20 µg | $410.00 | |||
SERCA1 Double Nickase Plasmid (h2) | sc-401416-NIC-2 | 20 µg | $410.00 |
ATP2A1 encodes the sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase SERCA1, a P-type ATPase that pumps cytosolic Ca²⁺ into the sarcoplasmic reticulum to restore low resting Ca²⁺ following excitation. By driving Ca²⁺ sequestration, SERCA1 shapes excitation–contraction coupling, Ca²⁺-dependent signaling, and ER/SR Ca²⁺ homeostasis that influences proteostasis and stress responses. SERCA1 function integrates with pathways controlling calcium cycling and muscle energetics, including coupling to ryanodine receptor-mediated Ca²⁺ release and calsequestrin buffering. Altered ATP2A1 activity or expression has been linked to skeletal muscle dysfunction phenotypes, making it a useful target for mechanistic studies of calcium handling and myofiber physiology.
SERCA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP2A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP2A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP2A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP2A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.