Selenoprotein P CRISPR/Cas9 KO Plasmid (bovine2): sc-437342

Overview

Selenoprotein P (SELENOP) is a secreted glycoprotein enriched in selenocysteine residues that functions as a principal selenium transport and storage factor in mammals, supporting systemic selenium distribution and redox homeostasis. It contributes to antioxidant defense by delivering selenium for selenoenzyme biosynthesis, influencing cellular responses to oxidative stress, inflammation, and endoplasmic reticulum stress. In bovine biology, SELENOP is relevant to hepatic secretion, vascular and metabolic regulation, and selenium-dependent immunity, linking selenium status to oxidative damage and inflammatory signaling. Dysregulated SELENOP expression or selenium availability is associated with altered redox signaling and metabolic imbalance, making it a useful molecular entry point for studying stress adaptation pathways and nutrient–gene interactions.

Selenoprotein P CRISPR/Cas9 KO Plasmid (bovine2) is a pool of plasmids designed for targeted disruption of the gene in bovine cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Selenoprotein P protein expression.

This CRISPR knockout system enables efficient generation of -deficient cell models for investigation of Selenoprotein P signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting exon(s) critical for Selenoprotein P function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Selenoprotein P CRISPR/Cas9 KO Plasmid (bovine) and Selenoprotein P CRISPR/Cas9 KO Plasmid (bovine2) target distinct sites within the locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Selenoprotein P HDR Plasmid (bovine) and Selenoprotein P HDR Plasmid (bovine2) contain a puromycin resistance cassette and an RFP reporter flanked by homology arms to support homology-directed repair at defined target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.