SCP-1 CRISPR/Cas9 KO Plasmid (m): sc-423231
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCP-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCP-1 CRISPR/Cas9 KO Plasmid (m) | sc-423231 | 20 µg | $397.00 |
Overview
Sycp1 encodes synaptonemal complex protein 1 (SCP-1), a meiosis-specific structural component of the synaptonemal complex that assembles along homologous chromosomes during prophase I. SCP-1 supports chromosome synapsis, promotes crossover formation, and helps coordinate recombination and DNA repair events required for faithful segregation in spermatocytes and oocytes. Disruption of SYCP1-dependent synapsis is linked to meiotic arrest and aneuploid gamete formation, making it relevant to studies of infertility and germ cell development. In mouse, Sycp1 is widely used as a marker and mechanistic node for dissecting meiotic progression, checkpoint signaling, and recombination pathway integrity.
SCP-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sycp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sycp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sycp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCP-1 protein expression.
This CRISPR knockout system enables efficient generation of Sycp1-deficient cell models for investigation of SCP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sycp1 exon(s) critical for SCP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sycp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCP-1 CRISPR/Cas9 KO Plasmid (m) and SCP-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sycp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCP-1 HDR Plasmid (m) and SCP-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sycp1 homology arms to support homology-directed repair at defined Sycp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.