SAK/STK18/PLK4 Antibody (19-Y7): sc-100413

PLK4 Antibody (19-Y7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PLK4 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). PLK4 (19-Y7) antibody is available as a non-conjugated format. PLK4, a member of the polo-like kinase family, plays a crucial role in cell cycle regulation, particularly during the transition from S phase to mitosis. A unique structure, characterized by a single polo box forming a dimer fold, enables PLK4 localization to key cellular structures such as nucleolus, centrosomes, and cleavage furrow. This localization ensures proper mitotic progression and accurate cell division. PLK4 expression gradually increases throughout the cell cycle with tightly regulated activity; dysregulation can lead to severe consequences, including increased apoptosis and anaphase arrest. Haploinsufficiency of PLK4 has been linked to spontaneous tumor development, particularly in the liver, highlighting PLK4′s importance in maintaining cellular integrity and preventing oncogenesis. PLK4′s ability to facilitate late mitotic progression and support embryonic development makes PLK4 critical for understanding cell proliferation and cancer biology.

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SAK/STK18/PLK4 Antibody (19-Y7) References:

1. Late mitotic failure in mice lacking Sak, a polo-like kinase.  |  Hudson, JW., et al. 2001. Curr Biol. 11: 441-6. PMID: 11301255
2. Polo-like kinase-2 is required for centriole duplication in mammalian cells.  |  Warnke, S., et al. 2004. Curr Biol. 14: 1200-7. PMID: 15242618
3. Differential regulation of polo-like kinase 1, 2, 3, and 4 gene expression in mammalian cells and tissues.  |  Winkles, JA. and Alberts, GF. 2005. Oncogene. 24: 260-6. PMID: 15640841
4. The Plk3-Cdc25 circuit.  |  Myer, DL., et al. 2005. Oncogene. 24: 299-305. PMID: 15640846
5. Sak/Plk4 and mitotic fidelity.  |  Swallow, CJ., et al. 2005. Oncogene. 24: 306-12. PMID: 15640847
6. SAK, a new polo-like kinase, is transcriptionally repressed by p53 and induces apoptosis upon RNAi silencing.  |  Li, J., et al. 2005. Neoplasia. 7: 312-23. PMID: 15967108
7. Plk4 haploinsufficiency causes mitotic infidelity and carcinogenesis.  |  Ko, MA., et al. 2005. Nat Genet. 37: 883-8. PMID: 16025114
8. The Polo kinase Plk4 functions in centriole duplication.  |  Habedanck, R., et al. 2005. Nat Cell Biol. 7: 1140-6. PMID: 16244668
9. TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.  |  Meitinger, F., et al. 2020. Nature. 585: 440-446. PMID: 32908304
10. Sak, a murine protein-serine/threonine kinase that is related to the Drosophila polo kinase and involved in cell proliferation.  |  Fode, C., et al. 1994. Proc Natl Acad Sci U S A. 91: 6388-92. PMID: 8022793

The preferred SAK/STK18/PLK4 antibody is SAK/STK18/PLK4 Antibody (D-6): sc-518222.