S-100A16 Lentiviral Activation Particles (h): sc-406149-LAC

S100A16 encodes S-100A16, a small EF-hand calcium-binding protein in the S100 family that contributes to Ca2+-dependent signaling and modulation of protein–protein interactions. S-100A16 has been linked to regulation of cell differentiation, cytoskeletal dynamics, and membrane-associated processes, and is frequently studied in the context of stress responses and metabolic remodeling. Altered S100A16 expression patterns have been reported across multiple tumor types and in cardiometabolic and inflammatory settings, supporting its use as a marker and functional node in disease-relevant pathways. As a calcium sensor-like protein, S-100A16 can influence transcriptional programs indirectly through signaling networks that couple calcium flux to changes in cell state.

S-100A16 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient S100A16 upregulation across a broader range of human cell types.

S-100A16 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the S100A16 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous S-100A16 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native S100A16 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.