RNF40 CRISPR/Cas9 KO Plasmid (h): sc-404156
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF40 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RNF40 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RNF40 Antibody (1C1): sc-293333
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF40 CRISPR/Cas9 KO Plasmid (h) | sc-404156 | 20 µg | $397.00 |
Overview
RNF40 encodes a RING finger E3 ubiquitin ligase that forms an obligate heterodimer with RNF20 to monoubiquitinate histone H2B (H2BK120ub), a key chromatin modification linking transcriptional elongation with epigenetic crosstalk to H3K4 and H3K79 methylation. Through this activity, RNF40 regulates RNA polymerase II–dependent gene expression programs, chromatin accessibility, and coordinated DNA damage responses that rely on chromatin remodeling at sites of replication stress or double-strand breaks. RNF40-dependent H2B ubiquitination impacts cell-cycle control and lineage-specific transcriptional networks, making it relevant to studies of genome stability and dysregulated transcription observed in cancer and other proliferative disorders. Altered RNF40 signaling has also been connected to changes in inflammatory and developmental pathways via context-dependent control of enhancer and promoter function.
RNF40 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RNF40 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RNF40 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RNF40 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RNF40 protein expression.
This CRISPR knockout system enables efficient generation of RNF40-deficient cell models for investigation of RNF40 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RNF40 exon(s) critical for RNF40 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RNF40 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RNF40 CRISPR/Cas9 KO Plasmid (h) and RNF40 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RNF40 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RNF40 HDR Plasmid (h) and RNF40 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RNF40 homology arms to support homology-directed repair at defined RNF40 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.