Rim3 CRISPR/Cas9 KO Plasmid (h): sc-407285
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rim3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rim3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rim3 CRISPR/Cas9 KO Plasmid (h) | sc-407285 | 20 µg | $397.00 |
Overview
RIMS3 encodes Rab3-interacting molecule 3 (Rim3), a presynaptic scaffolding protein that participates in organizing the neurotransmitter release apparatus at active zones. Through interactions with Rab3 GTPases and other synaptic components, Rim3 contributes to vesicle docking/priming, calcium-triggered exocytosis, and maintenance of synaptic efficacy. RIMS3-associated mechanisms intersect with neuronal signaling pathways governing synaptic transmission and plasticity, processes frequently implicated in neurodevelopmental and neuropsychiatric phenotypes. Altered expression or variation in RIMS3 has been investigated in the context of nervous system dysfunction, making it relevant for studying molecular determinants of synapse organization and network activity.
Rim3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RIMS3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RIMS3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RIMS3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rim3 protein expression.
This CRISPR knockout system enables efficient generation of RIMS3-deficient cell models for investigation of Rim3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RIMS3 exon(s) critical for Rim3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RIMS3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rim3 CRISPR/Cas9 KO Plasmid (h) and Rim3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RIMS3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rim3 HDR Plasmid (h) and Rim3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RIMS3 homology arms to support homology-directed repair at defined RIMS3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.