Protein L Agarose

Gebrauch :

Binding Capacity: ~5-10mg human IgG/mL of resin.

Storage: Upon receipt store product at 4-8°C. Product is shipped at ambient temperature.

Important Product Information
• Protein L only binds to immunoglobulins containing light chains of type kappa I, III, and IV in human and kappa I in mouse. Protein L also may be specific for certain kappa subgroups in other species. Protein L binds scfv without interfering with antigen binding.
• Protein L binds weakly to rabbit immunoglobulins and does not bind immunoglobulins from bovine, goat or sheep; nor does it bind to lambda light chains.
• The total IgG content of serum is approximately 10-15 mg/ml and, therefore, 2 ml of settled resin has only enough binding capacity to purify antibody from ~1 ml of serum. The concentration of antibody in tissue culture supernatant varies considerably among hybridoma clones.
• The described purification procedure is for human IgG. For optimal recovery, use a sample size such that the expected Ig load on the column is less than 80% of the maximum binding capacity.
• Serum samples, ascites fluid, plasma or tissue culture supernatant may be used with this product.

Erscheinung :

Suspension

Aggregatzustand :

Liquid

Lagerung :

Store at 4° C