PP2B-Aα Antibody (D-9): sc-17808

PP2B-Aα Antibody (D-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects PP2B-A alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PP2B-A alpha antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2B-A alpha protein, also known as calcineurin A alpha, plays a crucial role in calcium-dependent signaling pathways by dephosphorylating serine and threonine residues on target proteins, which is essential for regulating numerous cellular processes such as T-cell activation, muscle contraction, and neuronal signaling. PP2B-A alpha is primarily located in the cytoplasm and is activated by calcium-bound calmodulin, highlighting PP2B-A alpha′s importance in translating calcium signals into cellular responses. PP2B-A alpha interaction with calmodulin is vital for enzymatic activity, and dysregulation of this interaction can lead to various pathophysiological conditions, including autoimmune diseases and cardiac dysfunction. Using PP2B-Aα (D-9) monoclonal antibody in research can provide valuable insights into regulatory mechanisms of calcium signaling and implications in health and disease.

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PP2B-Aα Antibody (D-9) References:

1. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
2. Calcineurin-A alpha activation enhances the structure and function of regenerating muscles after myotoxic injury.  |  Stupka, N., et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293: R686-94. PMID: 17475677
3. Cellular and molecular consequences of calcineurin A alpha gene deletion.  |  Sun, L., et al. 2007. Ann N Y Acad Sci. 1116: 216-26. PMID: 17872390
4. Calcineurin A-α suppression drives nuclear factor-κB-mediated NADPH oxidase-2 upregulation.  |  Cheriyan, AM., et al. 2021. Am J Physiol Renal Physiol. 320: F789-F798. PMID: 33615888
5. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
6. Characterization of a mutant calcineurin A alpha gene expressed by EL4 lymphoma cells.  |  Fruman, DA., et al. 1995. Mol Cell Biol. 15: 3857-63. PMID: 7791792
7. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
8. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
9. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
10. Molecular cloning of a full-length cDNA encoding the catalytic subunit of human calmodulin-dependent protein phosphatase (calcineurin A alpha).  |  Muramatsu, T. and Kincaid, RL. 1993. Biochim Biophys Acta. 1178: 117-20. PMID: 8392375
11. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923
12. Insights derived from the structures of the Ser/Thr phosphatases calcineurin and protein phosphatase 1.  |  Lohse, DL., et al. 1995. Structure. 3: 987-90. PMID: 8590008

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2B-Aα Antibody (D-9) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2B-Aα Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2B-Aα Antibody (D-9) Specifications

Alternate Product Name: PPP3CA

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 312-521

Epitope Target: PP2B-Aα

Epitope Species: human

Product Reactivity/Detection: PP2B-Aα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for PP2B-Aα Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin