Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PP1γ CRISPR/Cas9 KO Plasmid (m2): sc-422379-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP1γ CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PP1γ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PP1γ Antibody (E-4): sc-515943
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP1γ CRISPR/Cas9 KO Plasmid (m2) | sc-422379-KO-2 | 20 µg | $397.00 |
Overview
Ppp1cc encodes the mouse protein phosphatase 1 gamma (PP1γ), a catalytic serine/threonine phosphatase that controls phosphorylation-dependent signaling by dephosphorylating diverse substrates in the nucleus and cytoplasm. PP1γ functions within multiprotein holoenzymes that direct specificity toward processes including cell-cycle progression, chromatin dynamics, RNA processing, and cytoskeletal remodeling. Through these pathways, PP1γ contributes to regulation of mitotic exit, DNA damage responses, and cellular differentiation programs. Dysregulated PP1-family signaling and altered phosphorylation homeostasis are linked to mechanisms relevant to proliferative disorders, genomic instability, and neurobiology, supporting its use in pathway-focused functional studies.
PP1γ CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Ppp1cc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppp1cc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppp1cc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PP1γ protein expression.
This CRISPR knockout system enables efficient generation of Ppp1cc-deficient cell models for investigation of PP1γ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppp1cc exon(s) critical for PP1γ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppp1cc genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PP1γ CRISPR/Cas9 KO Plasmid (m) and PP1γ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppp1cc locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PP1γ HDR Plasmid (m) and PP1γ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppp1cc homology arms to support homology-directed repair at defined Ppp1cc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.