Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Phox2b CRISPR/Cas9 KO Plasmid (h): sc-401351
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Phox2b CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Phox2b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Phox2b Antibody (B-11): sc-376997
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Phox2b CRISPR/Cas9 KO Plasmid (h) | sc-401351 | 20 µg | $397.00 |
Overview
PHOX2B encodes Phox2b, a paired-like homeobox transcription factor that orchestrates autonomic nervous system development and noradrenergic neuronal differentiation by controlling gene expression programs during neural crest and hindbrain patterning. Phox2b regulates transcriptional networks involved in catecholamine biosynthesis, neuronal specification, and maturation, interfacing with pathways that shape neural progenitor fate decisions and peripheral nervous system lineage commitment. Altered PHOX2B function is strongly associated with neurocristopathies, including congenital central hypoventilation syndrome, and with developmental tumors of neural crest origin such as neuroblastoma, reflecting its role in establishing and maintaining autonomic neuronal identity. As a lineage-defining regulator, PHOX2B serves as a useful node for studying transcriptional control of neurodevelopment and mechanisms of cell-state stability and reprogramming.
Phox2b CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHOX2B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHOX2B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHOX2B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Phox2b protein expression.
This CRISPR knockout system enables efficient generation of PHOX2B-deficient cell models for investigation of Phox2b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHOX2B exon(s) critical for Phox2b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHOX2B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Phox2b CRISPR/Cas9 KO Plasmid (h) and Phox2b CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHOX2B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Phox2b HDR Plasmid (h) and Phox2b HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHOX2B homology arms to support homology-directed repair at defined PHOX2B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.